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Protein concentrations were determined using a BCA protein assay (Thermo Fisher Scientific) and 60 g of protein/lane were separated by SDSPAGE using e-PAGEL (ATTO Co., Tokyo, Japan) and were electro transferred onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories Inc., Hercules, CA) for 30 min at 25 V

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View study Bock-Marquette I, Saxena A, White MD, DiMaio JM, Srivastava D

Steady-state levels are reached over the first several weeks, and most people are evaluating appetite and progress over months of consistent weekly use, not days